When the Node.js application starts, create a new connection and utilise the old database connection object:
/server.js
import express from 'express';
import Promise from 'bluebird';
import logger from 'winston';
import { MongoClient } from 'mongodb';
import config from './config';
import usersRestApi from './api/users';
const app = express();
app.use('/api/users', usersRestApi);
app.get('/', (req, res) => {
  res.send('Hello World');
});
// Create a MongoDB connection pool and start the application
// after the database connection is ready
MongoClient.connect(config.database.url, { promiseLibrary: Promise }, (err, db) => {
  if (err) {
    logger.warn(`Failed to connect to the database. ${err.stack}`);
  }
  app.locals.db = db;
  app.listen(config.port, () => {
    logger.info(`Node.js app is listening at http://localhost:${config.port}`);
  });
});
/api/users.js
import { Router } from 'express';
import { ObjectID } from 'mongodb';
const router = new Router();
router.get('/:id', async (req, res, next) => {
  try {
    const db = req.app.locals.db;
    const id = new ObjectID(req.params.id);
    const user = await db.collection('user').findOne({ _id: id }, {
      email: 1,
      firstName: 1,
      lastName: 1
    });
    if (user) {
      user.id = req.params.id;
      res.send(user);
    } else {
      res.sendStatus(404);
    }
  } catch (err) {
    next(err);
  }
});
export default router;
Source: How to Open Database Connections in a Node.js/Express App
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